Measurement of the second osmotic virial coefficient for protein solutions exhibiting monomer–dimer equilibrium
نویسندگان
چکیده
منابع مشابه
Why is the osmotic second virial coefficient related to protein crystallization?
A molecular basis is presented for characterizing the osmotic second virial coefficient, B 22 , of dilute protein solutions, which provides a measure of the nature of protein—protein interactions and has been shown to be correlated with crystallization behavior. Experimental measurements of the second virial coefficient of lysozyme and bovine a-chymotrypsinogen A were performed by static light ...
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Intermolecular potential energy surface (IPS) for protein — protein has been examined using RHF, DFT-B3LYPand MP2 levels of theory with 6-31G, 6-31G* basis sets. A number of basis sets were used in order to evaluatethe basis set effects, at all three levels of theory, basis sets has significant effects on the calculated potentialenergy curves (including position, depth and width of the potentia...
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Membrane osmometry is used to measure osmotic pressures of dilute solutions containing quasi-spherical CdSe nanocrystals covered with polymer brushes in toluene in the range 31 to 45°C. Osmotic-pressure data, as a function of nanocrystal concentration, yield the molecular weight and the osmotic second virial coefficient of the nanocrystals; the latter is related to the potential of mean force b...
متن کاملApplications of the second virial coefficient: protein crystallization and solubility
A number of citations in the article by Wilson & DeLucas [(2014). Acta Cryst. F70, 543-554] are corrected.
متن کاملNonmonotonic variation with salt concentration of the second virial coefficient in protein solutions.
The osmotic virial coefficient B2 of globular protein solutions is calculated as a function of added salt concentration at fixed pH by computer simulations of the "primitive model." The salt and counterions as well as a discrete charge pattern on the protein surface are explicitly incorporated. For parameters roughly corresponding to lysozyme, we find that B2 first decreases with added salt con...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 2008
ISSN: 0003-2697
DOI: 10.1016/j.ab.2008.03.032